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Method Article
Pranela Rameshwar
Rameshwar Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The tumorsphere assay allows one to assess whether a single cell harbors the potential to both initiate and maintain tumors in the absence of cellular interaction and adhesion. Stem cells are capable of unlimited self-renewal ability, and unique subsets of cancer cells that acquire stem cell properties have the theoretical ability to form de novo tumors when grown under low-attachment conditions within minimal growth factor supplementation. Primary stem cells and progenitor cells from the breast can be enriched within mammospheres, a concept that is analogous to neural stem cell enrichment in neurospheres. Tumorigenic efficiency of a subset of cancer cells can be determined based on number of spheres that emerge from single cells. On the contrary, cancer cells that lack stem cell properties have limited sphere-forming potential due to telomere loss and cellular senescence.
Keywords
Tumorsphere Passage for Breast Cancer Stem Cells
No conflicting interests.
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Associated Publications
Delineation of breast cancer cell hierarchy identifies the subset responsible for dormancy
by Shyam A. Patel, Shakti H. Ramkissoon, Margarette Bryan, +6
Scientific Reports (05 February, 2013)
Method Article
Pranela Rameshwar
Rameshwar Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 11 Feb, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The tumorsphere assay allows one to assess whether a single cell harbors the potential to both initiate and maintain tumors in the absence of cellular interaction and adhesion. Stem cells are capable of unlimited self-renewal ability, and unique subsets of cancer cells that acquire stem cell properties have the theoretical ability to form de novo tumors when grown under low-attachment conditions within minimal growth factor supplementation. Primary stem cells and progenitor cells from the breast can be enriched within mammospheres, a concept that is analogous to neural stem cell enrichment in neurospheres. Tumorigenic efficiency of a subset of cancer cells can be determined based on number of spheres that emerge from single cells. On the contrary, cancer cells that lack stem cell properties have limited sphere-forming potential due to telomere loss and cellular senescence.
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Associated Publications
Delineation of breast cancer cell hierarchy identifies the subset responsible for dormancy
by Shyam A. Patel, Shakti H. Ramkissoon, Margarette Bryan, +6
Scientific Reports (05 February, 2013)