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Method Article
Pranela Rameshwar
Rameshwar Lab
Corresponding Author
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The noble agar assay is based on the principle that the cancer cells are anchorage-independent and do not show contact inhibition. This type of soft agar assay can give insight into two important properties of a subset of cancer cells. Firstly, the tumorigenic efficiency of cancer cells can be determined based on number of colonies that emerge. This is based on the ability of cells to grow unattached and to remain suspended in agar. Secondly, the self-renewal properties of cancer cells can be assessed based on the types of progeny produced in noble agar.
Keywords
self-renewal, tumorigenicity
Figure 1
No conflicting interests.
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Associated Publications
Delineation of breast cancer cell hierarchy identifies the subset responsible for dormancy
by Shyam A. Patel, Shakti H. Ramkissoon, Margarette Bryan, +6
Scientific Reports (05 February, 2013)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Pranela Rameshwar
Rameshwar Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 11 Feb, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The noble agar assay is based on the principle that the cancer cells are anchorage-independent and do not show contact inhibition. This type of soft agar assay can give insight into two important properties of a subset of cancer cells. Firstly, the tumorigenic efficiency of cancer cells can be determined based on number of colonies that emerge. This is based on the ability of cells to grow unattached and to remain suspended in agar. Secondly, the self-renewal properties of cancer cells can be assessed based on the types of progeny produced in noble agar.
Figure 1
No conflicting interests.
Loading...
Associated Publications
Delineation of breast cancer cell hierarchy identifies the subset responsible for dormancy
by Shyam A. Patel, Shakti H. Ramkissoon, Margarette Bryan, +6
Scientific Reports (05 February, 2013)