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Method Article
Direct imaging furin-controlled intracellular condensation with two-photon laser microscope
Gaolin Liang
CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China
Corresponding Author
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We developed a new “smart” Eu-based probe (2) which is susceptive to furin, a protease overexpressed in cancer cells. Upon furin cleavage, 2 condenses to form oligomers and the latter self-assemble into Europium nanparticles (Eu-NPs) on site. Two-photon laser microscopy (TPLM) imaging of MDA-MB-468 cells incubated with 2 showed strong fluorescence signals from Golgi networks, suggesting 2 was under the action of furin and trapped at/near the locations of furin (i.e., Golgi networks). TPLM imaging of MDA-MB-468 cells incubated with the scrambled control of 2 (i.e., 2-Scr) at same condition only exhibits uniform, weak fluorescence signals. These results suggest that 2 could be a useful probe for TPLM imaging of furin activity in cancer cells. We describe herein a detailed protocol of cell preparation and TPLM imaging with 2.
Keywords
two-photon laser microscopy, europium, condensation reaction
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Associated Publications
Controlled intracellular self-assembly of gadolinium nanoparticles as smart molecular MR contrast agents
by Chun-Yan Cao, Ying-Ying Shen, Jian-Dong Wang, +2
Scientific Reports (03 January, 2013)
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This is a preprint. It has not completed peer review.
Method Article
Direct imaging furin-controlled intracellular condensation with two-photon laser microscope
Gaolin Liang
CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 Jan, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We developed a new “smart” Eu-based probe (2) which is susceptive to furin, a protease overexpressed in cancer cells. Upon furin cleavage, 2 condenses to form oligomers and the latter self-assemble into Europium nanparticles (Eu-NPs) on site. Two-photon laser microscopy (TPLM) imaging of MDA-MB-468 cells incubated with 2 showed strong fluorescence signals from Golgi networks, suggesting 2 was under the action of furin and trapped at/near the locations of furin (i.e., Golgi networks). TPLM imaging of MDA-MB-468 cells incubated with the scrambled control of 2 (i.e., 2-Scr) at same condition only exhibits uniform, weak fluorescence signals. These results suggest that 2 could be a useful probe for TPLM imaging of furin activity in cancer cells. We describe herein a detailed protocol of cell preparation and TPLM imaging with 2.
Figure 1
Figure 2
Figure 3
Associated Publications
Controlled intracellular self-assembly of gadolinium nanoparticles as smart molecular MR contrast agents
by Chun-Yan Cao, Ying-Ying Shen, Jian-Dong Wang, +2
Scientific Reports (03 January, 2013)