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Method Article
Isolation, culture and transplantation of female germline stem cells from neonatal and prepubertal mice
Ji Wu
Key laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, 200240, China
Corresponding Author
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The successful isolation and culture of female germline stem cells (FGSCs) that exist in postnatal and adult mammals will allow us to study their biological characteristics and their applications in biotechnology and medicine. In this study, we describe systematic procedures for the culture and transplantation of FGSCs. To establish mouse FGSC lines, the FGSCs were isolated from the ovaries of postnatal mice by enzymatic digestion and immunomagnetic purification. After culture, FGSCs were transplanted into the ovaries of recipient females that were sterilized by chemotherapy to analyze physiological function. Establishment of FGSC lines allows new possibilities for the use of FGSCs in biotechnology and medicine.
Keywords
female germline stem cell, isolation, purification, culture
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Table 1 Primers used for investigation of genes specific for FGSCs
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Table 2 Troubleshooting table
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Associated Publications
Production of offspring from a germline stem cell line derived from neonatal ovaries
by Kang Zou, Zhe Yuan, Zhaojuan Yang, +9
Nature Cell Biology (28 December, 2012)
Improved Efficiency of Female Germline Stem Cell Purification Using Fragilis-Based Magnetic Bead Sorting
by Kang Zou, Lin Hou, Kejing Sun, +2
Stem Cells And Development (28 December, 2012)
Production of transgenic mice by random recombination of targeted genes in female germline stem cells
by Y. Zhang, Z. Yang, Y. Yang, +9
Journal Of Molecular Cell Biology (28 December, 2012)
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This is a preprint. It has not completed peer review.
Method Article
Isolation, culture and transplantation of female germline stem cells from neonatal and prepubertal mice
Ji Wu
Key laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, 200240, China
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 02 Jan, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The successful isolation and culture of female germline stem cells (FGSCs) that exist in postnatal and adult mammals will allow us to study their biological characteristics and their applications in biotechnology and medicine. In this study, we describe systematic procedures for the culture and transplantation of FGSCs. To establish mouse FGSC lines, the FGSCs were isolated from the ovaries of postnatal mice by enzymatic digestion and immunomagnetic purification. After culture, FGSCs were transplanted into the ovaries of recipient females that were sterilized by chemotherapy to analyze physiological function. Establishment of FGSC lines allows new possibilities for the use of FGSCs in biotechnology and medicine.
Figure 1
Figure 2
Figure 3
Associated Publications
Production of offspring from a germline stem cell line derived from neonatal ovaries
by Kang Zou, Zhe Yuan, Zhaojuan Yang, +9
Nature Cell Biology (28 December, 2012)
Improved Efficiency of Female Germline Stem Cell Purification Using Fragilis-Based Magnetic Bead Sorting
by Kang Zou, Lin Hou, Kejing Sun, +2
Stem Cells And Development (28 December, 2012)
Production of transgenic mice by random recombination of targeted genes in female germline stem cells
by Y. Zhang, Z. Yang, Y. Yang, +9
Journal Of Molecular Cell Biology (28 December, 2012)