Isolation, culture and transplantation of female germline stem cells from neonatal and prepubertal mice
The successful isolation and culture of female germline stem cells (FGSCs) that exist in postnatal and adult mammals will allow us to study their biological characteristics and their applications in biotechnology and medicine. In this study, we describe systematic procedures for the culture and transplantation of FGSCs. To establish mouse FGSC lines, the FGSCs were isolated from the ovaries of postnatal mice by enzymatic digestion and immunomagnetic purification. After culture, FGSCs were transplanted into the ovaries of recipient females that were sterilized by chemotherapy to analyze physiological function. Establishment of FGSC lines allows new possibilities for the use of FGSCs in biotechnology and medicine.
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Table 1 Primers used for investigation of genes specific for FGSCs
Table 2 Troubleshooting table
Posted 02 Jan, 2013
Isolation, culture and transplantation of female germline stem cells from neonatal and prepubertal mice
Posted 02 Jan, 2013
The successful isolation and culture of female germline stem cells (FGSCs) that exist in postnatal and adult mammals will allow us to study their biological characteristics and their applications in biotechnology and medicine. In this study, we describe systematic procedures for the culture and transplantation of FGSCs. To establish mouse FGSC lines, the FGSCs were isolated from the ovaries of postnatal mice by enzymatic digestion and immunomagnetic purification. After culture, FGSCs were transplanted into the ovaries of recipient females that were sterilized by chemotherapy to analyze physiological function. Establishment of FGSC lines allows new possibilities for the use of FGSCs in biotechnology and medicine.
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