Fluorescent 2 color Whole mount in situ hybridization for a mouse embryo

doi:10.1038/protex.2013.002

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Method Article

Tetsuya Nakamura, Hiroshi Hamada

Tetsuya Nakamura

Culver Hall 106 , Department of Organismal Biology and Anatomy,  University of Chicago,  900 E. 57th St. Chicago, IL 60637

Corresponding Author

Hiroshi Hamada

Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan

DOI:

10.1038/protex.2013.002

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New developed fluorescent 2 color Whole mount in situ hybridization produces a clear and high S/N ratio image to analyze mRNA localization inside 1 cell and in tissue levels.

Keywords

in situ hybridization, fluorescence

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Associated Publications

Fluid flow and interlinked feedback loops establish left–right asymmetric decay of Cerl2 mRNA
by Tetsuya Nakamura, Daisuke Saito, Aiko Kawasumi, +8
Nature Communications (10 December, 2012)

An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

Tetsuya Nakamura, Hiroshi Hamada

Tetsuya Nakamura

Culver Hall 106 , Department of Organismal Biology and Anatomy,  University of Chicago,  900 E. 57th St. Chicago, IL 60637

Corresponding Author

Hiroshi Hamada

Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan

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CURRENT STATUS: Posted

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Cell biology

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Molecular Biology

DOI:

10.1038/protex.2013.002

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The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

New developed fluorescent 2 color Whole mount in situ hybridization produces a clear and high S/N ratio image to analyze mRNA localization inside 1 cell and in tissue levels.

Figure 1

No.

Associated Publications

Fluid flow and interlinked feedback loops establish left–right asymmetric decay of Cerl2 mRNA
by Tetsuya Nakamura, Daisuke Saito, Aiko Kawasumi, +8
Nature Communications (10 December, 2012)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Tetsuya Nakamura, Hiroshi Hamada

Tetsuya Nakamura

Culver Hall 106 , Department of Organismal Biology and Anatomy, University of Chicago, 900 E. 57th St. Chicago, IL 60637

Corresponding Author

Hiroshi Hamada

Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan

DOI:

10.1038/protex.2013.002

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

in situ hybridization, fluorescence

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Anticipated Results

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Acknowledgements

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Biological techniques

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Associated Publications

Fluid flow and interlinked feedback loops establish left–right asymmetric decay of Cerl2 mRNA

by Tetsuya Nakamura, Daisuke Saito, Aiko Kawasumi, +8

Nature Communications (10 December, 2012)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Tetsuya Nakamura, Hiroshi Hamada

Tetsuya Nakamura

Culver Hall 106 , Department of Organismal Biology and Anatomy, University of Chicago, 900 E. 57th St. Chicago, IL 60637

Corresponding Author

Hiroshi Hamada

Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan

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Molecular Biology

DOI & PDF

DOI:

10.1038/protex.2013.002

Download PDF

License

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

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Abstract

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Introduction

Culver Hall 106 , Department of Organismal Biology and Anatomy,  University of Chicago,  900 E. 57th St. Chicago, IL 60637

Culver Hall 106 , Department of Organismal Biology and Anatomy,  University of Chicago,  900 E. 57th St. Chicago, IL 60637