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Method Article
Asis Datta
National Institute of Plant Genome Research, New Delhi, India
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
Gas chromatography- mass spectrometry (GC-MS) is a highly used and well established technological platform for metabolite profiling in both plant and non-plant species. Here we describe a simple protocol for lipophilic compounds extraction and analysis from tomato fruits and leaves which is an important crop in terms of its economic and nutritional value. This is a rapid, reliable and reproducible method to detect and quantify lipophilic metabolites including fatty acids, fatty alcohols, sterols, alkanes, alkenes, and triterpenoids by GC-MS. In addition, this is a simple and convenient method for efficient comparative lipid profiling of wild type and transgenic or mutant tomato fruits.
Keywords
Gas chromatography-mass spectrometry, metabolomics, lipophilic
The authors declare no competing financial interests
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.doc
Table1 Troubleshooting
- supplement0.doc
Table 2 Major lipophilic metabolites detected by GC-MS
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Associated Publications
Expression of a fungal sterol desaturase improves tomato drought tolerance, pathogen resistance and nutritional quality
by Ayushi Kamthan, Mohan Kamthan, Mohammad Azam, +3
Scientific Reports (11 December, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Asis Datta
National Institute of Plant Genome Research, New Delhi, India
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 13 Dec, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Gas chromatography- mass spectrometry (GC-MS) is a highly used and well established technological platform for metabolite profiling in both plant and non-plant species. Here we describe a simple protocol for lipophilic compounds extraction and analysis from tomato fruits and leaves which is an important crop in terms of its economic and nutritional value. This is a rapid, reliable and reproducible method to detect and quantify lipophilic metabolites including fatty acids, fatty alcohols, sterols, alkanes, alkenes, and triterpenoids by GC-MS. In addition, this is a simple and convenient method for efficient comparative lipid profiling of wild type and transgenic or mutant tomato fruits.
The authors declare no competing financial interests
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.doc
Table1 Troubleshooting
- supplement0.doc
Table 2 Major lipophilic metabolites detected by GC-MS
Loading...
Associated Publications
Expression of a fungal sterol desaturase improves tomato drought tolerance, pathogen resistance and nutritional quality
by Ayushi Kamthan, Mohan Kamthan, Mohammad Azam, +3
Scientific Reports (11 December, 2012)