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Method Article
A simple protocol for extraction, derivatization, and analysis of tomato leaf and fruit lipophilic metabolites using GC-MS
Asis Datta
National Institute of Plant Genome Research, New Delhi, India
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
Gas chromatography- mass spectrometry (GC-MS) is a highly used and well established technological platform for metabolite profiling in both plant and non-plant species. Here we describe a simple protocol for lipophilic compounds extraction and analysis from tomato fruits and leaves which is an important crop in terms of its economic and nutritional value. This is a rapid, reliable and reproducible method to detect and quantify lipophilic metabolites including fatty acids, fatty alcohols, sterols, alkanes, alkenes, and triterpenoids by GC-MS. In addition, this is a simple and convenient method for efficient comparative lipid profiling of wild type and transgenic or mutant tomato fruits.
Keywords
Gas chromatography-mass spectrometry, metabolomics, lipophilic
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Table1 Troubleshooting
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Table 2 Major lipophilic metabolites detected by GC-MS
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Associated Publications
Expression of a fungal sterol desaturase improves tomato drought tolerance, pathogen resistance and nutritional quality
by Ayushi Kamthan, Mohan Kamthan, Mohammad Azam, +3
Scientific Reports (11 December, 2012)
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This is a preprint. It has not completed peer review.
Method Article
A simple protocol for extraction, derivatization, and analysis of tomato leaf and fruit lipophilic metabolites using GC-MS
Asis Datta
National Institute of Plant Genome Research, New Delhi, India
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Dec, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Gas chromatography- mass spectrometry (GC-MS) is a highly used and well established technological platform for metabolite profiling in both plant and non-plant species. Here we describe a simple protocol for lipophilic compounds extraction and analysis from tomato fruits and leaves which is an important crop in terms of its economic and nutritional value. This is a rapid, reliable and reproducible method to detect and quantify lipophilic metabolites including fatty acids, fatty alcohols, sterols, alkanes, alkenes, and triterpenoids by GC-MS. In addition, this is a simple and convenient method for efficient comparative lipid profiling of wild type and transgenic or mutant tomato fruits.
Associated Publications
Expression of a fungal sterol desaturase improves tomato drought tolerance, pathogen resistance and nutritional quality
by Ayushi Kamthan, Mohan Kamthan, Mohammad Azam, +3
Scientific Reports (11 December, 2012)