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Method Article
Baojun Li
State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The protocol reported here describes an optical method for flexible manipulation of particles and cells. Using a tapered optical fibre probe launched with a laser at 980-nm wavelength, particles and cells can be stably trapped, targeted driven to designated positions, and flexibly arranged into desired patterns. This method provides a highly potential avenue for highly flexible and precise manipulation of biological objects.
Keywords
Optical manipulation, particles, yeast cells, Escherichia coli bacteria
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The authors declare no competing financial interests.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.mov
Supplementary Video 4 *Detailed process of driving six particles to form a triangle.* <iframe width="480" height="360" src="http://www.youtube.com/embed/XVgx30j8w6Y?rel=0" frameborder="0" allowfullscreen></iframe>
- supplement0.mov
Supplementary Video 1 *Optical trapping of a particle.* <iframe width="480" height="360" src="http://www.youtube.com/embed/UqzIAyeFHQc?rel=0" frameborder="0" allowfullscreen></iframe>
- supplement0.mov
Supplementary Video 2 *Optical driving of a particle.* <iframe width="480" height="360" src="http://www.youtube.com/embed/74Uei1PWJI0?rel=0" frameborder="0" allowfullscreen></iframe>
- supplement0.mov
Supplementary Video 3 *Detailed process of pick-and-position of the sixth particle to form the sixth vertex of the hexagon.* <iframe width="480" height="360" src="http://www.youtube.com/embed/DFcGG3T9hNU?rel=0" frameborder="0" allowfullscreen></iframe>
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Posted 12 Nov, 2012
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Associated Publications
Optical trapping, driving, and arrangement of particles using a tapered fibre probe
by Hongbao Xin, Rui Xu, and Baojun Li
Scientific Reports (12 November, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Baojun Li
State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510275, China
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Nov, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The protocol reported here describes an optical method for flexible manipulation of particles and cells. Using a tapered optical fibre probe launched with a laser at 980-nm wavelength, particles and cells can be stably trapped, targeted driven to designated positions, and flexibly arranged into desired patterns. This method provides a highly potential avenue for highly flexible and precise manipulation of biological objects.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
The authors declare no competing financial interests.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.mov
Supplementary Video 4 *Detailed process of driving six particles to form a triangle.* <iframe width="480" height="360" src="http://www.youtube.com/embed/XVgx30j8w6Y?rel=0" frameborder="0" allowfullscreen></iframe>
- supplement0.mov
Supplementary Video 1 *Optical trapping of a particle.* <iframe width="480" height="360" src="http://www.youtube.com/embed/UqzIAyeFHQc?rel=0" frameborder="0" allowfullscreen></iframe>
- supplement0.mov
Supplementary Video 2 *Optical driving of a particle.* <iframe width="480" height="360" src="http://www.youtube.com/embed/74Uei1PWJI0?rel=0" frameborder="0" allowfullscreen></iframe>
- supplement0.mov
Supplementary Video 3 *Detailed process of pick-and-position of the sixth particle to form the sixth vertex of the hexagon.* <iframe width="480" height="360" src="http://www.youtube.com/embed/DFcGG3T9hNU?rel=0" frameborder="0" allowfullscreen></iframe>
Loading...
Associated Publications
Optical trapping, driving, and arrangement of particles using a tapered fibre probe
by Hongbao Xin, Rui Xu, and Baojun Li
Scientific Reports (12 November, 2012)