Procedure for reductive methylation of protein to improve crystallizability

doi:10.1038/nprot.2007.287

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Method Article

Neil Shaw, Chongyun Cheng, Zhi-Jie Liu

Neil Shaw

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Chongyun Cheng

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Zhi-Jie Liu

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

DOI:

10.1038/nprot.2007.287

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Biochemistry

Biological techniques

Structural biology

Associated Publications

The multifunctional human p100 protein “hooks” methylated ligands
by Neil Shaw, Min Zhao, Chongyun Cheng, +9
Nature Structural & Molecular Biology (31 May, 2007)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Neil Shaw, Chongyun Cheng, Zhi-Jie Liu

Neil Shaw

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Chongyun Cheng

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Zhi-Jie Liu

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Version History

CURRENT STATUS: Posted

Version 1

Posted 20 Jul, 2007

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Subject Areas

Biochemistry

Biological techniques

Structural biology

DOI:

10.1038/nprot.2007.287

Download PDF

The full text of this article is available to read as a PDF.

Associated Publications

The multifunctional human p100 protein “hooks” methylated ligands
by Neil Shaw, Min Zhao, Chongyun Cheng, +9
Nature Structural & Molecular Biology (31 May, 2007)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Neil Shaw, Chongyun Cheng, Zhi-Jie Liu

Neil Shaw

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Chongyun Cheng

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Zhi-Jie Liu

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

DOI:

10.1038/nprot.2007.287

Download PDF

Introduction

Reagents

Equipment

Procedure

Timing

Critical Steps

Troubleshooting

Anticipated Results

References

Acknowledgements

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Subject Areas

Biochemistry

Biological techniques

Structural biology

Associated Publications

The multifunctional human p100 protein “hooks” methylated ligands

by Neil Shaw, Min Zhao, Chongyun Cheng, +9

Nature Structural & Molecular Biology (31 May, 2007)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Neil Shaw, Chongyun Cheng, Zhi-Jie Liu

Neil Shaw

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Chongyun Cheng

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Zhi-Jie Liu

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

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DOI & PDF

DOI:

10.1038/nprot.2007.287

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License

Declarations

Introduction

Reagents

Equipment

Procedure

Timing

Critical Steps

Troubleshooting

Anticipated Results

References

Acknowledgements