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Method Article
Total Nucleic Acid Extraction from Soil
Roey Angel
Max Planck Institute for Terrestrial Microbiology
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
The following protocol is intended for the simultaneous extraction of DNA and RNA from various soil samples along with suggestions on how to tweak the protocol for soil with higher humic content. The protocol has been used by many and results in very high yields of nucleic acids, typically much more than commercial kits. For buffers and solutions used in this protocol, please see accompanying document Buffers and Solutions for TNA Extractions.pdf.
Keywords
DNA, RNA, mRNA, Soil, Extraction, Humic acids
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Solutions for TNA Extraction Solutions for TNA Extraction
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Associated Publications
Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions
by Roey Angel, Peter Claus, and Ralf Conrad
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This is a preprint. It has not completed peer review.
Method Article
Total Nucleic Acid Extraction from Soil
Roey Angel
Max Planck Institute for Terrestrial Microbiology
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Oct, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The following protocol is intended for the simultaneous extraction of DNA and RNA from various soil samples along with suggestions on how to tweak the protocol for soil with higher humic content. The protocol has been used by many and results in very high yields of nucleic acids, typically much more than commercial kits. For buffers and solutions used in this protocol, please see accompanying document Buffers and Solutions for TNA Extractions.pdf.
Associated Publications
Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions
by Roey Angel, Peter Claus, and Ralf Conrad