Total Nucleic Acid Extraction from Soil
The following protocol is intended for the simultaneous extraction of DNA and RNA from various soil samples along with suggestions on how to tweak the protocol for soil with higher humic content. The protocol has been used by many and results in very high yields of nucleic acids, typically much more than commercial kits. For buffers and solutions used in this protocol, please see accompanying document Buffers and Solutions for TNA Extractions.pdf.
This is a list of supplementary files associated with this preprint. Click to download.
Solutions for TNA Extraction Solutions for TNA Extraction
Posted 23 Oct, 2012
Total Nucleic Acid Extraction from Soil
Posted 23 Oct, 2012
The following protocol is intended for the simultaneous extraction of DNA and RNA from various soil samples along with suggestions on how to tweak the protocol for soil with higher humic content. The protocol has been used by many and results in very high yields of nucleic acids, typically much more than commercial kits. For buffers and solutions used in this protocol, please see accompanying document Buffers and Solutions for TNA Extractions.pdf.
© Research Square 2021