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Method Article
Pierre Brat
UMR QualiSud, CIRAD Monptellier, Montpellier, France
Corresponding Author
Alain Robichon
UMR7254 INRA/CNRS/UNS, Institut Sophia Agrobiotech, Sophia Antipolis, France
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
Carotenoids are compounds synthesized in plants, bacteria and fungi, closely associated to the chlorophyll to perform photosynthesis. A spectacular evolutionary achievement allowed the aphid to produce carotenoids obviously by lateral transfer of genes from fungi. We have recently documented that these molecules are involved in photo conditioning of metabolism such ATP synthesis in this insect model. The carotenoid synthesis in aphid was directly determined in living insects without extraction procedure by Raman imaging technique and the analysis was compared between insects raised in distinct environments. In contrast with the Raman spectrum that provides information on molecular motifs recognition (conjugated double bonds) but does not discriminate between isomers and ester forms, the mass spectrometry technology allowed us to analyze finely the variations in carotenoid composition. In parallel to these spectral analysis and chemistry determination, series of dosage of ATP were performed in order to correlate carotenoid synthesis and metabolism in aphid.
Keywords
aphid, carotene, Raman spectroscopy, ATP, mass spectrometry
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The authors declare no competing financial interests.
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Associated Publications
Light- induced electron transfer and ATP synthesis in a carotene synthesizing insect
by Jean Christophe Valmalette, Aviv Dombrovsky, Pierre Brat, +3
Scientific Reports (04 October, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Pierre Brat
UMR QualiSud, CIRAD Monptellier, Montpellier, France
Corresponding Author
Alain Robichon
UMR7254 INRA/CNRS/UNS, Institut Sophia Agrobiotech, Sophia Antipolis, France
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 10 Oct, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Carotenoids are compounds synthesized in plants, bacteria and fungi, closely associated to the chlorophyll to perform photosynthesis. A spectacular evolutionary achievement allowed the aphid to produce carotenoids obviously by lateral transfer of genes from fungi. We have recently documented that these molecules are involved in photo conditioning of metabolism such ATP synthesis in this insect model. The carotenoid synthesis in aphid was directly determined in living insects without extraction procedure by Raman imaging technique and the analysis was compared between insects raised in distinct environments. In contrast with the Raman spectrum that provides information on molecular motifs recognition (conjugated double bonds) but does not discriminate between isomers and ester forms, the mass spectrometry technology allowed us to analyze finely the variations in carotenoid composition. In parallel to these spectral analysis and chemistry determination, series of dosage of ATP were performed in order to correlate carotenoid synthesis and metabolism in aphid.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
The authors declare no competing financial interests.
Loading...
Associated Publications
Light- induced electron transfer and ATP synthesis in a carotene synthesizing insect
by Jean Christophe Valmalette, Aviv Dombrovsky, Pierre Brat, +3
Scientific Reports (04 October, 2012)