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Method Article
Analysis of carotenoid compounds in aphids by Raman imaging and mass spectrometry
Pierre Brat
UMR QualiSud, CIRAD Monptellier, Montpellier, France
Corresponding Author
Alain Robichon
UMR7254 INRA/CNRS/UNS, Institut Sophia Agrobiotech, Sophia Antipolis, France
Corresponding Author
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Carotenoids are compounds synthesized in plants, bacteria and fungi, closely associated to the chlorophyll to perform photosynthesis. A spectacular evolutionary achievement allowed the aphid to produce carotenoids obviously by lateral transfer of genes from fungi. We have recently documented that these molecules are involved in photo conditioning of metabolism such ATP synthesis in this insect model. The carotenoid synthesis in aphid was directly determined in living insects without extraction procedure by Raman imaging technique and the analysis was compared between insects raised in distinct environments. In contrast with the Raman spectrum that provides information on molecular motifs recognition (conjugated double bonds) but does not discriminate between isomers and ester forms, the mass spectrometry technology allowed us to analyze finely the variations in carotenoid composition. In parallel to these spectral analysis and chemistry determination, series of dosage of ATP were performed in order to correlate carotenoid synthesis and metabolism in aphid.
Keywords
aphid, carotene, Raman spectroscopy, ATP, mass spectrometry
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Associated Publications
Light- induced electron transfer and ATP synthesis in a carotene synthesizing insect
by Jean Christophe Valmalette, Aviv Dombrovsky, Pierre Brat, +3
Scientific Reports (04 October, 2012)
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This is a preprint. It has not completed peer review.
Method Article
Analysis of carotenoid compounds in aphids by Raman imaging and mass spectrometry
Pierre Brat
UMR QualiSud, CIRAD Monptellier, Montpellier, France
Corresponding Author
Alain Robichon
UMR7254 INRA/CNRS/UNS, Institut Sophia Agrobiotech, Sophia Antipolis, France
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Oct, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Carotenoids are compounds synthesized in plants, bacteria and fungi, closely associated to the chlorophyll to perform photosynthesis. A spectacular evolutionary achievement allowed the aphid to produce carotenoids obviously by lateral transfer of genes from fungi. We have recently documented that these molecules are involved in photo conditioning of metabolism such ATP synthesis in this insect model. The carotenoid synthesis in aphid was directly determined in living insects without extraction procedure by Raman imaging technique and the analysis was compared between insects raised in distinct environments. In contrast with the Raman spectrum that provides information on molecular motifs recognition (conjugated double bonds) but does not discriminate between isomers and ester forms, the mass spectrometry technology allowed us to analyze finely the variations in carotenoid composition. In parallel to these spectral analysis and chemistry determination, series of dosage of ATP were performed in order to correlate carotenoid synthesis and metabolism in aphid.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Associated Publications
Light- induced electron transfer and ATP synthesis in a carotene synthesizing insect
by Jean Christophe Valmalette, Aviv Dombrovsky, Pierre Brat, +3
Scientific Reports (04 October, 2012)