An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Hisashi Moriguchi
Moriguichi H Lab, The University of Tokyo and Harvard Medical School
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
THE EDITORS ARE RETRACTING THIS _PROTOCOL EXCHANGE_ PROTOCOL BECAUSE THE PAPER UPON WHICH IT IS BASED HAS BEEN RETRACTED.
The development of new method to cryopreserve human ovarian cortex tissues without damage is needed for the improvement of quality of life(QOL) of female cancer patients. Here we show novel cryopreservation method of human ovarian cortex tissues by using supercooling (S.C.) procedure. In our study, we have found that the stable expressions of 9 genes within human oocytes by using our S.C. procedure are necessary in order to produce human mature eggs and experience pregnancy with high safety profiles by In vitro fertilisation (IVF). Our method will be helpful in order to preserve fertility of female cancer patients.
Keywords
Supercooling, cryopreservation of human ovarian cortex tissues, human oocytes, human mature eggs
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
We have no conflicting financial interests.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 27 Jul, 2012
Community comments: 8
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Successful cryopreservation of human ovarian cortex tissues using supercooling
by Hisashi Moriguchi, Yue Zhang, Makoto Mihara, +1
Scientific Reports (21 July, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Hisashi Moriguchi
Moriguichi H Lab, The University of Tokyo and Harvard Medical School
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 27 Jul, 2012
Community comments: 8
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
THE EDITORS ARE RETRACTING THIS _PROTOCOL EXCHANGE_ PROTOCOL BECAUSE THE PAPER UPON WHICH IT IS BASED HAS BEEN RETRACTED.
The development of new method to cryopreserve human ovarian cortex tissues without damage is needed for the improvement of quality of life(QOL) of female cancer patients. Here we show novel cryopreservation method of human ovarian cortex tissues by using supercooling (S.C.) procedure. In our study, we have found that the stable expressions of 9 genes within human oocytes by using our S.C. procedure are necessary in order to produce human mature eggs and experience pregnancy with high safety profiles by In vitro fertilisation (IVF). Our method will be helpful in order to preserve fertility of female cancer patients.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
We have no conflicting financial interests.
Loading...
Associated Publications
Successful cryopreservation of human ovarian cortex tissues using supercooling
by Hisashi Moriguchi, Yue Zhang, Makoto Mihara, +1
Scientific Reports (21 July, 2012)