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Method Article
Successful cryopreservation of human ovarian cortex tissues using supercooling
Hisashi Moriguchi
Moriguichi H Lab, The University of Tokyo and Harvard Medical School
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
THE EDITORS ARE RETRACTING THIS _PROTOCOL EXCHANGE_ PROTOCOL BECAUSE THE PAPER UPON WHICH IT IS BASED HAS BEEN RETRACTED.
The development of new method to cryopreserve human ovarian cortex tissues without damage is needed for the improvement of quality of life(QOL) of female cancer patients. Here we show novel cryopreservation method of human ovarian cortex tissues by using supercooling (S.C.) procedure. In our study, we have found that the stable expressions of 9 genes within human oocytes by using our S.C. procedure are necessary in order to produce human mature eggs and experience pregnancy with high safety profiles by In vitro fertilisation (IVF). Our method will be helpful in order to preserve fertility of female cancer patients.
Keywords
Supercooling, cryopreservation of human ovarian cortex tissues, human oocytes, human mature eggs
Figure 1
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Figure 5
commented on 12 October, 2012
Editorial Note: Protocol Exchange has become aware of irregularities related to authors affiliations and the statement describing IRB approval in this protocol. The situation is being investigated.
commented on 16 October, 2012
Editorial Note: Sigeo Ihara, Yoshihiro Ohta and Yue Zhang have been removed from the author list at their request.The author list has changed from: Hisashi Moriguchi, Yue Zhang, Sigeo Ihara, Yoshihiro Ohta & Chifumi Satoto: Hisashi Moriguchi & Chifumi Sato
commented on 16 October, 2012
Editorial Note: Chifumi Sato has been removed from the author list at his request.The author list has changed from: Hisashi Moriguchi & Chifumi Satoto: Hisashi Moriguchi
commented on 08 November, 2012
Editorial Note: The above protocol was part of a research paper entitled "Successful cryopreservation of human ovarian cortex tissues using supercooling" which was published in Scientific Reports on 27 July 2012. The research paper has now been retracted - for further information on the retraction please go to http://www.nature.com/srep/2012/121108/srep00840/full/srep00840.html.
commented on 12 October, 2012
Editorial Note: Protocol Exchange has become aware of irregularities related to authors affiliations and the statement describing IRB approval in this protocol. The situation is being investigated.
commented on 16 October, 2012
Editorial Note: Sigeo Ihara, Yoshihiro Ohta and Yue Zhang have been removed from the author list at their request.The author list has changed from: Hisashi Moriguchi, Yue Zhang, Sigeo Ihara, Yoshihiro Ohta & Chifumi Satoto: Hisashi Moriguchi & Chifumi Sato
commented on 16 October, 2012
Editorial Note: Chifumi Sato has been removed from the author list at his request.The author list has changed from: Hisashi Moriguchi & Chifumi Satoto: Hisashi Moriguchi
commented on 08 November, 2012
Editorial Note: The above protocol was part of a research paper entitled "Successful cryopreservation of human ovarian cortex tissues using supercooling" which was published in Scientific Reports on 27 July 2012. The research paper has now been retracted - for further information on the retraction please go to http://www.nature.com/srep/2012/121108/srep00840/full/srep00840.html.
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Associated Publications
Successful cryopreservation of human ovarian cortex tissues using supercooling
by Hisashi Moriguchi, Yue Zhang, Makoto Mihara, +1
Scientific Reports (21 July, 2012)
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This is a preprint. It has not completed peer review.
Method Article
Successful cryopreservation of human ovarian cortex tissues using supercooling
Hisashi Moriguchi
Moriguichi H Lab, The University of Tokyo and Harvard Medical School
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 Jul, 2012
Community comments: 8
Metrics
Comments: 8
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
THE EDITORS ARE RETRACTING THIS _PROTOCOL EXCHANGE_ PROTOCOL BECAUSE THE PAPER UPON WHICH IT IS BASED HAS BEEN RETRACTED.
The development of new method to cryopreserve human ovarian cortex tissues without damage is needed for the improvement of quality of life(QOL) of female cancer patients. Here we show novel cryopreservation method of human ovarian cortex tissues by using supercooling (S.C.) procedure. In our study, we have found that the stable expressions of 9 genes within human oocytes by using our S.C. procedure are necessary in order to produce human mature eggs and experience pregnancy with high safety profiles by In vitro fertilisation (IVF). Our method will be helpful in order to preserve fertility of female cancer patients.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
commented on 12 October, 2012
Editorial Note: Protocol Exchange has become aware of irregularities related to authors affiliations and the statement describing IRB approval in this protocol. The situation is being investigated.
commented on 16 October, 2012
Editorial Note: Sigeo Ihara, Yoshihiro Ohta and Yue Zhang have been removed from the author list at their request.The author list has changed from: Hisashi Moriguchi, Yue Zhang, Sigeo Ihara, Yoshihiro Ohta & Chifumi Satoto: Hisashi Moriguchi & Chifumi Sato
commented on 16 October, 2012
Editorial Note: Chifumi Sato has been removed from the author list at his request.The author list has changed from: Hisashi Moriguchi & Chifumi Satoto: Hisashi Moriguchi
commented on 08 November, 2012
Editorial Note: The above protocol was part of a research paper entitled "Successful cryopreservation of human ovarian cortex tissues using supercooling" which was published in Scientific Reports on 27 July 2012. The research paper has now been retracted - for further information on the retraction please go to http://www.nature.com/srep/2012/121108/srep00840/full/srep00840.html.
commented on 12 October, 2012
Editorial Note: Protocol Exchange has become aware of irregularities related to authors affiliations and the statement describing IRB approval in this protocol. The situation is being investigated.
commented on 16 October, 2012
Editorial Note: Sigeo Ihara, Yoshihiro Ohta and Yue Zhang have been removed from the author list at their request.The author list has changed from: Hisashi Moriguchi, Yue Zhang, Sigeo Ihara, Yoshihiro Ohta & Chifumi Satoto: Hisashi Moriguchi & Chifumi Sato
commented on 16 October, 2012
Editorial Note: Chifumi Sato has been removed from the author list at his request.The author list has changed from: Hisashi Moriguchi & Chifumi Satoto: Hisashi Moriguchi
commented on 08 November, 2012
Editorial Note: The above protocol was part of a research paper entitled "Successful cryopreservation of human ovarian cortex tissues using supercooling" which was published in Scientific Reports on 27 July 2012. The research paper has now been retracted - for further information on the retraction please go to http://www.nature.com/srep/2012/121108/srep00840/full/srep00840.html.
2 comments flagged for moderation
If you posted a comment and think it was mistakenly removed, please contact customer support at [email protected].
Associated Publications
Successful cryopreservation of human ovarian cortex tissues using supercooling
by Hisashi Moriguchi, Yue Zhang, Makoto Mihara, +1
Scientific Reports (21 July, 2012)