In vitro enzymatic activity assay for ENOLASE in mammalian cells in culture
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
Posted 21 Aug, 2012
In vitro enzymatic activity assay for ENOLASE in mammalian cells in culture
Posted 21 Aug, 2012
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
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