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Method Article
Florian Muller
Ronald DePinho
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
Keywords
Enolase, Enzyme, Activity, Spectrophotometry, Cell Culture
None
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Associated Publications
Passenger deletions generate therapeutic vulnerabilities in cancer
by Florian L. Muller, Simona Colla, Elisa Aquilanti, +14
Nature (22 July, 2012)
Method Article
Florian Muller
Ronald DePinho
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 21 Aug, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
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Associated Publications
Passenger deletions generate therapeutic vulnerabilities in cancer
by Florian L. Muller, Simona Colla, Elisa Aquilanti, +14
Nature (22 July, 2012)