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Method Article
Florian Muller
Ronald DePinho
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
Keywords
Enolase, Enzyme, Activity, Spectrophotometry, Cell Culture
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Associated Publications
Passenger deletions generate therapeutic vulnerabilities in cancer
by Florian L. Muller, Simona Colla, Elisa Aquilanti, +14
Nature (22 July, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Florian Muller
Ronald DePinho
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 21 Aug, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
None
Loading...
Associated Publications
Passenger deletions generate therapeutic vulnerabilities in cancer
by Florian L. Muller, Simona Colla, Elisa Aquilanti, +14
Nature (22 July, 2012)