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Method Article
In vitro enzymatic activity assay for ENOLASE in mammalian cells in culture
Florian Muller
Ronald DePinho
Corresponding Author
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Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
Keywords
Enolase, Enzyme, Activity, Spectrophotometry, Cell Culture
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Associated Publications
Passenger deletions generate therapeutic vulnerabilities in cancer
by Florian L. Muller, Simona Colla, Elisa Aquilanti, +14
Nature (22 July, 2012)
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This is a preprint. It has not completed peer review.
Method Article
In vitro enzymatic activity assay for ENOLASE in mammalian cells in culture
Florian Muller
Ronald DePinho
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 21 Aug, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Enolase (EC 4.2.1.11) is the second to last step in glycolysis and the third step in gluconeogenesis, catalyzing the reversible conversation of 2-phosphoglycerate and phosphoenolpyruvate. Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity is of interest to investigators in diverse fields, including those studying cancer cell metabolism. We describe a simple and rapid in vitro protocol to measure Enolase activity in native lysates from mammalian cells in culture.
Associated Publications
Passenger deletions generate therapeutic vulnerabilities in cancer
by Florian L. Muller, Simona Colla, Elisa Aquilanti, +14
Nature (22 July, 2012)