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Method Article
Sippy Kaur
Butzow's Lab, University of Helsinki
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Deregulation of microRNA (miRNA) is found in various cancers and knowledge regarding their spatial expression pattern is a key point for determining their biological role and for understanding the complexity of various regulatory networks. Here we present protocol for fast and sensitive chromogenic miRNA-in situ hybridization (ISH) for analyzing paraffin embedded tissue microarray (TMA). This protocol is based on combination of locked nucleic acid (LNA) probes and chromogenic detection system. Particular conditions for proteinase treatment are a prerequisite for specific and sensitive staining and in this protocol proteinase K treatment for TMA was optimised This protocol gave successful signals with various probes and tissues, thereby enabling the miRNA detection in wide range of cancers using TMA.
Keywords
microRNA, Tissue microarrays, Chromogenic in situ detection ,
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Posted 22 Jun, 2012
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Biological techniques
Associated Publications
MiR-34a Expression Has an Effect for Lower Risk of Metastasis and Associates with Expression Patterns Predicting Clinical Outcome in Breast Cancer
by Hanna Peurala, Dario Greco, Tuomas Heikkinen, +10
PLoS ONE (15 June, 2012)
A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer
by N. Li, S. Kaur, J. Greshock, +13
Cancer Research (15 June, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Sippy Kaur
Butzow's Lab, University of Helsinki
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 22 Jun, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Deregulation of microRNA (miRNA) is found in various cancers and knowledge regarding their spatial expression pattern is a key point for determining their biological role and for understanding the complexity of various regulatory networks. Here we present protocol for fast and sensitive chromogenic miRNA-in situ hybridization (ISH) for analyzing paraffin embedded tissue microarray (TMA). This protocol is based on combination of locked nucleic acid (LNA) probes and chromogenic detection system. Particular conditions for proteinase treatment are a prerequisite for specific and sensitive staining and in this protocol proteinase K treatment for TMA was optimised This protocol gave successful signals with various probes and tissues, thereby enabling the miRNA detection in wide range of cancers using TMA.
Figure 1
Figure 2
no
Loading...
Associated Publications
MiR-34a Expression Has an Effect for Lower Risk of Metastasis and Associates with Expression Patterns Predicting Clinical Outcome in Breast Cancer
by Hanna Peurala, Dario Greco, Tuomas Heikkinen, +10
PLoS ONE (15 June, 2012)
A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer
by N. Li, S. Kaur, J. Greshock, +13
Cancer Research (15 June, 2012)