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Method Article
Probabilistic mapping and image clustering for quantitative assessment of fluorescent protein localizations in Arabidopsis guard cells
Takumi Higaki
Graduate School of Frontier Sciences, The University of Tokyo
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The protocol reported here describes a method to quantitatively evaluate fluorescently-tagged protein localizations from fluorescent microscopic images with a combination of probabilistic mapping and image clustering. We demonstrate the use of this protocol using kidney-shaped guard cells of plants.
Keywords
Image processing, Microscopy, Protein localization, Organelle distribution, Plant stomata
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Subject Areas
Cell biology
Computational biology and bioinformatics
Biological techniques
Plant sciences
Associated Publications
Statistical organelle dissection of Arabidopsis guard cells using image database LIPS
by Takumi Higaki, Natsumaro Kutsuna, Yoichiroh Hosokawa, +5
Scientific Reports (21 May, 2012)
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This is a preprint. It has not completed peer review.
Method Article
Probabilistic mapping and image clustering for quantitative assessment of fluorescent protein localizations in Arabidopsis guard cells
Takumi Higaki
Graduate School of Frontier Sciences, The University of Tokyo
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 21 May, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The protocol reported here describes a method to quantitatively evaluate fluorescently-tagged protein localizations from fluorescent microscopic images with a combination of probabilistic mapping and image clustering. We demonstrate the use of this protocol using kidney-shaped guard cells of plants.
Figure 1
Figure 2
Figure 3
Figure 4
Associated Publications
Statistical organelle dissection of Arabidopsis guard cells using image database LIPS
by Takumi Higaki, Natsumaro Kutsuna, Yoichiroh Hosokawa, +5
Scientific Reports (21 May, 2012)