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Method Article
Takumi Higaki
Graduate School of Frontier Sciences, The University of Tokyo
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The protocol reported here describes a method to quantitatively evaluate fluorescently-tagged protein localizations from fluorescent microscopic images with a combination of probabilistic mapping and image clustering. We demonstrate the use of this protocol using kidney-shaped guard cells of plants.
Keywords
Image processing, Microscopy, Protein localization, Organelle distribution, Plant stomata
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The authors declare no competing financial interests.
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Posted 21 May, 2012
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Subject Areas
Cell biology
Computational biology and bioinformatics
Biological techniques
Plant sciences
Associated Publications
Statistical organelle dissection of Arabidopsis guard cells using image database LIPS
by Takumi Higaki, Natsumaro Kutsuna, Yoichiroh Hosokawa, +5
Scientific Reports (21 May, 2012)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Takumi Higaki
Graduate School of Frontier Sciences, The University of Tokyo
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 21 May, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The protocol reported here describes a method to quantitatively evaluate fluorescently-tagged protein localizations from fluorescent microscopic images with a combination of probabilistic mapping and image clustering. We demonstrate the use of this protocol using kidney-shaped guard cells of plants.
Figure 1
Figure 2
Figure 3
Figure 4
The authors declare no competing financial interests.
Loading...
Associated Publications
Statistical organelle dissection of Arabidopsis guard cells using image database LIPS
by Takumi Higaki, Natsumaro Kutsuna, Yoichiroh Hosokawa, +5
Scientific Reports (21 May, 2012)