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Method Article
Hamid Kalantari
Cyto's Lab (Royan Institute)
Corresponding Author
Hamid Gourabi
President of Royan Institute
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Our observation showed the addition of cytochalasin-B to mouse embryonic stem cells (mESC) culture for CBMn analysis led to the induction of apoptosis in these cells. On the other hand, addition of cyt-B is the most critical part of the cytokinesis-block micronucleus assay (CBMn) technique that cannot be omitted. Thus, modification of the traditional CBMn assay seems to be necessary. In this paper, we attempt to troubleshoot this problem and show that the CBMn assay can be used in embryonic stem cells research, particularly as a reliable tool in monitoring the cytogenetic integrity of cells. The potential reasons of the observed induction of apoptosis in mESCs are also discussed.
The full text pdf version of this protocol can be accessed "here":http://www.nature.com/protocolexchange/system/uploads/2107/original/protex.2012.011_-_full_text.pdf?1334222191
Keywords
micronucleus cytome assay , apoptosis, stem cell, cytochalasin-B
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The authors declare no competing financial interests.
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Full protocol Adapted cytokinesis-block micronucleus assay (CBMn) for mouse embryonic stem cells
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Method Article
Hamid Kalantari
Cyto's Lab (Royan Institute)
Corresponding Author
Hamid Gourabi
President of Royan Institute
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Apr, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Our observation showed the addition of cytochalasin-B to mouse embryonic stem cells (mESC) culture for CBMn analysis led to the induction of apoptosis in these cells. On the other hand, addition of cyt-B is the most critical part of the cytokinesis-block micronucleus assay (CBMn) technique that cannot be omitted. Thus, modification of the traditional CBMn assay seems to be necessary. In this paper, we attempt to troubleshoot this problem and show that the CBMn assay can be used in embryonic stem cells research, particularly as a reliable tool in monitoring the cytogenetic integrity of cells. The potential reasons of the observed induction of apoptosis in mESCs are also discussed.
The full text pdf version of this protocol can be accessed "here":http://www.nature.com/protocolexchange/system/uploads/2107/original/protex.2012.011_-_full_text.pdf?1334222191
Figure 1
Figure 2
Figure 3
Figure 4
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