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Method Article
Magnetic assisted transfection of primary neurons
Flavie Sicard
OZBiosciences
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Magnetofection™ is a novel, simple and highly efficient method to transfect cells in vitro and in vivo. This technology exploits magnetic force to drive the nucleic acids associated with magnetic particles towards and into the target cells. In this way, the complete applied dose of nucleic acids gets concentrated on the cells within few minutes so that 100% of the cells get in contact simultaneously with all DNA or RNA doses. NeuroMag is a magnetic nanoparticles formulation specifically designed to achieve high transfection efficiency of primary neurons and neuronal cell lines. In this way, NeuroMag is the ideal transfection reagent for neurosciences applications.
NeuroMag has been developed for very efficient transfections of various types of nucleic acids such as DNA, RNA or oligonucleotides in primary neurons and immortalized neuronal cells. It is particularly efficient with co-culture of primary hippocampal or corticoid neurons. NeuroMag is serum compatible and can be used for transient and stable transfection. This product is very stable, ready-to-use and intended for research purpose only.
Keywords
Primary Neurons Transfection
Mélanie Bertuzzi
commented on 04 April, 2012
Cyclin D1 Induction Preceding Neuronal Death via the Excitotoxic NMDA Pathway Involves Selective Stimulation of Extrasynaptic NMDA Receptors and JNK Pathway. Efthimiadi L, Farso M, Quirion R, Krantic S 10.1159/000335911
commented on 23 April, 2012
On the 24th of April 2012, the title of this protocol changed from: "Magnetofection using NeuroMag" to "Magnetic assisted transfection of primary neurons."
Mélanie Bertuzzi
commented on 04 April, 2012
Cyclin D1 Induction Preceding Neuronal Death via the Excitotoxic NMDA Pathway Involves Selective Stimulation of Extrasynaptic NMDA Receptors and JNK Pathway. Efthimiadi L, Farso M, Quirion R, Krantic S 10.1159/000335911
commented on 23 April, 2012
On the 24th of April 2012, the title of this protocol changed from: "Magnetofection using NeuroMag" to "Magnetic assisted transfection of primary neurons."
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CURRENT STATUS: Posted
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Posted 05 Apr, 2012
Community comments: 4
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Subject Areas
Cell biology
Nanoscience and technology
Neuroscience
Chemical biology
Biological techniques
Genetics
Molecular Biology
Associated Publications
Magnetic Nanoparticles Enhance Adenovirus Transduction In Vitro and In Vivo
by Cédric Sapet, Christophe Pellegrino, Nicolas Laurent, +2
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This is a preprint. It has not completed peer review.
Method Article
Magnetic assisted transfection of primary neurons
Flavie Sicard
OZBiosciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Apr, 2012
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Magnetofection™ is a novel, simple and highly efficient method to transfect cells in vitro and in vivo. This technology exploits magnetic force to drive the nucleic acids associated with magnetic particles towards and into the target cells. In this way, the complete applied dose of nucleic acids gets concentrated on the cells within few minutes so that 100% of the cells get in contact simultaneously with all DNA or RNA doses. NeuroMag is a magnetic nanoparticles formulation specifically designed to achieve high transfection efficiency of primary neurons and neuronal cell lines. In this way, NeuroMag is the ideal transfection reagent for neurosciences applications.
NeuroMag has been developed for very efficient transfections of various types of nucleic acids such as DNA, RNA or oligonucleotides in primary neurons and immortalized neuronal cells. It is particularly efficient with co-culture of primary hippocampal or corticoid neurons. NeuroMag is serum compatible and can be used for transient and stable transfection. This product is very stable, ready-to-use and intended for research purpose only.
Mélanie Bertuzzi
commented on 04 April, 2012
Cyclin D1 Induction Preceding Neuronal Death via the Excitotoxic NMDA Pathway Involves Selective Stimulation of Extrasynaptic NMDA Receptors and JNK Pathway. Efthimiadi L, Farso M, Quirion R, Krantic S 10.1159/000335911
commented on 23 April, 2012
On the 24th of April 2012, the title of this protocol changed from: "Magnetofection using NeuroMag" to "Magnetic assisted transfection of primary neurons."
Mélanie Bertuzzi
commented on 04 April, 2012
Cyclin D1 Induction Preceding Neuronal Death via the Excitotoxic NMDA Pathway Involves Selective Stimulation of Extrasynaptic NMDA Receptors and JNK Pathway. Efthimiadi L, Farso M, Quirion R, Krantic S 10.1159/000335911
commented on 23 April, 2012
On the 24th of April 2012, the title of this protocol changed from: "Magnetofection using NeuroMag" to "Magnetic assisted transfection of primary neurons."
Associated Publications
Magnetic Nanoparticles Enhance Adenovirus Transduction In Vitro and In Vivo
by Cédric Sapet, Christophe Pellegrino, Nicolas Laurent, +2