This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Standardized Relative Quantification of Immunofluorescence Tissue Staining
Stephan Tenbaum
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Héctor G. Palmer
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The detection of correlations between the expression levels or sub-cellular localization of different proteins with specific characteristics of human tumors, such as e.g. grade of malignancy, may give important hints of functional associations. Here we describe the method we use for relative quantification of immunofluorescence staining of tumor tissue sections, which allows us to compare between different samples based on objective data. With this strategy we have quantified, for instance, the amounts of nuclear FOXO3a and Beta-Catenin proteins in human colon carcinomas.
Keywords
Immunofluorescence, quantification of protein amounts, protein localization, confocal microscopy
Figure 1
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 02 Apr, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
Associated Publications
SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity
by A Barbáchano, P Ordóñez-Morán, J M García, +10
Oncogene (30 March, 2012)
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Standardized Relative Quantification of Immunofluorescence Tissue Staining
Stephan Tenbaum
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Héctor G. Palmer
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 02 Apr, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The detection of correlations between the expression levels or sub-cellular localization of different proteins with specific characteristics of human tumors, such as e.g. grade of malignancy, may give important hints of functional associations. Here we describe the method we use for relative quantification of immunofluorescence staining of tumor tissue sections, which allows us to compare between different samples based on objective data. With this strategy we have quantified, for instance, the amounts of nuclear FOXO3a and Beta-Catenin proteins in human colon carcinomas.
Figure 1
Associated Publications
SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity
by A Barbáchano, P Ordóñez-Morán, J M García, +10
Oncogene (30 March, 2012)