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Method Article
Stephan Tenbaum
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Héctor G. Palmer
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The detection of correlations between the expression levels or sub-cellular localization of different proteins with specific characteristics of human tumors, such as e.g. grade of malignancy, may give important hints of functional associations. Here we describe the method we use for relative quantification of immunofluorescence staining of tumor tissue sections, which allows us to compare between different samples based on objective data. With this strategy we have quantified, for instance, the amounts of nuclear FOXO3a and Beta-Catenin proteins in human colon carcinomas.
Keywords
Immunofluorescence, quantification of protein amounts, protein localization, confocal microscopy
Figure 1
Non declared
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Posted 02 Apr, 2012
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Associated Publications
SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity
by A Barbáchano, P Ordóñez-Morán, J M García, +10
Oncogene (30 March, 2012)
Method Article
Stephan Tenbaum
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Héctor G. Palmer
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 02 Apr, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The detection of correlations between the expression levels or sub-cellular localization of different proteins with specific characteristics of human tumors, such as e.g. grade of malignancy, may give important hints of functional associations. Here we describe the method we use for relative quantification of immunofluorescence staining of tumor tissue sections, which allows us to compare between different samples based on objective data. With this strategy we have quantified, for instance, the amounts of nuclear FOXO3a and Beta-Catenin proteins in human colon carcinomas.
Figure 1
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Associated Publications
SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity
by A Barbáchano, P Ordóñez-Morán, J M García, +10
Oncogene (30 March, 2012)