Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid nitrogen. Snap freezing achieves the same endpoint as slow rate-controlled freezing, but at approximate rate of -10-1000°C/min, compared to -1°C/min. Snap freezing with a CoolRack® module will provide sample vessel stability, organization and consistent freezing parameters, rapid hands-free sample processing while avoiding lost or contaminated samples. Snap freezing is performed on a pre-cooled CoolRack, which ensures fast heat transfer. This method can provide excellent specimen integrity and a wide array of options for analysis, including extraction of proteins, DNA and RNA for use in research and diagnostics. The following protocol describes a general procedure for cryopreserving bacteria for long-term storage. Verify with your laboratory SOP for specific needs for each strain.
Figure 1
All authors work for BioCision LLC, the manufacturer of the CoolRack used to freeze the bacteria
Loading...
Posted 08 Mar, 2012
Posted 08 Mar, 2012
Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid nitrogen. Snap freezing achieves the same endpoint as slow rate-controlled freezing, but at approximate rate of -10-1000°C/min, compared to -1°C/min. Snap freezing with a CoolRack® module will provide sample vessel stability, organization and consistent freezing parameters, rapid hands-free sample processing while avoiding lost or contaminated samples. Snap freezing is performed on a pre-cooled CoolRack, which ensures fast heat transfer. This method can provide excellent specimen integrity and a wide array of options for analysis, including extraction of proteins, DNA and RNA for use in research and diagnostics. The following protocol describes a general procedure for cryopreserving bacteria for long-term storage. Verify with your laboratory SOP for specific needs for each strain.
Figure 1
Loading...
© Research Square 2021