This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Bacteria Cryopreservation Protocol
Maria Thompson
BioCision
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid nitrogen. Snap freezing achieves the same endpoint as slow rate-controlled freezing, but at approximate rate of -10-1000°C/min, compared to -1°C/min. Snap freezing with a CoolRack® module will provide sample vessel stability, organization and consistent freezing parameters, rapid hands-free sample processing while avoiding lost or contaminated samples. Snap freezing is performed on a pre-cooled CoolRack, which ensures fast heat transfer. This method can provide excellent specimen integrity and a wide array of options for analysis, including extraction of proteins, DNA and RNA for use in research and diagnostics. The following protocol describes a general procedure for cryopreserving bacteria for long-term storage. Verify with your laboratory SOP for specific needs for each strain.
Keywords
bacteria, freezing
Figure 1
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Mar, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Bacteria Cryopreservation Protocol
Maria Thompson
BioCision
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Mar, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid nitrogen. Snap freezing achieves the same endpoint as slow rate-controlled freezing, but at approximate rate of -10-1000°C/min, compared to -1°C/min. Snap freezing with a CoolRack® module will provide sample vessel stability, organization and consistent freezing parameters, rapid hands-free sample processing while avoiding lost or contaminated samples. Snap freezing is performed on a pre-cooled CoolRack, which ensures fast heat transfer. This method can provide excellent specimen integrity and a wide array of options for analysis, including extraction of proteins, DNA and RNA for use in research and diagnostics. The following protocol describes a general procedure for cryopreserving bacteria for long-term storage. Verify with your laboratory SOP for specific needs for each strain.
Figure 1