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Method Article
Maria Thompson
BioCision
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid nitrogen. Snap freezing achieves the same endpoint as slow rate-controlled freezing, but at approximate rate of -10-1000°C/min, compared to -1°C/min. Snap freezing with a CoolRack® module will provide sample vessel stability, organization and consistent freezing parameters, rapid hands-free sample processing while avoiding lost or contaminated samples. Snap freezing is performed on a pre-cooled CoolRack, which ensures fast heat transfer. This method can provide excellent specimen integrity and a wide array of options for analysis, including extraction of proteins, DNA and RNA for use in research and diagnostics. The following protocol describes a general procedure for cryopreserving bacteria for long-term storage. Verify with your laboratory SOP for specific needs for each strain.
Keywords
bacteria, freezing
Figure 1
All authors work for BioCision LLC, the manufacturer of the CoolRack used to freeze the bacteria
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Posted 08 Mar, 2012
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Maria Thompson
BioCision
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 08 Mar, 2012
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid nitrogen. Snap freezing achieves the same endpoint as slow rate-controlled freezing, but at approximate rate of -10-1000°C/min, compared to -1°C/min. Snap freezing with a CoolRack® module will provide sample vessel stability, organization and consistent freezing parameters, rapid hands-free sample processing while avoiding lost or contaminated samples. Snap freezing is performed on a pre-cooled CoolRack, which ensures fast heat transfer. This method can provide excellent specimen integrity and a wide array of options for analysis, including extraction of proteins, DNA and RNA for use in research and diagnostics. The following protocol describes a general procedure for cryopreserving bacteria for long-term storage. Verify with your laboratory SOP for specific needs for each strain.
Figure 1
All authors work for BioCision LLC, the manufacturer of the CoolRack used to freeze the bacteria
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