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Method Article
Wagner Luiz Tafuri
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil.
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In dogs, a parasite burden is evident throughout the gastrointestinal tract but not is observed pathological alterations. Our aim was isolating lamina propria cells from dogs infected with leishmaniasis to further investigate if the parasite in the gut is correlated with the pathogenesis of the visceral infection. The biopsies were obtained of dogs and incubated with collagenase II. In the cells suspension was added antibodies against superficial and intracellular markers. Few studies of the markers used in this work have been done in the lamina propria cells in leishmaniasis. It is of great importance to determine markers in this tissue especially those who are described as being involved with the persistence of Leishmania infection within cells due to the fact that the infection in the gut does not induce lesions. Furthermore, work is ongoing in terms of investigating other segments of the GIT, a systematic study, from dogs with Leishmania
A pdf of the full version of this protocol can be found "here":http://www.nature.com/protocolexchange/system/uploads/2040/original/Fullprotocol-_protex.2011.273.pdf?1323950759
Keywords
Dog, Visceral Leishmaniasis, Flow Cytometer, Gut, Leishmania infantum
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We do not have any kind of commercial interesting or competition with other researches. It is only an academic manuscript. We have proposed a method that could improve immunological studies concerning canine gut mucosa as it involves isolating colonic lamina propria cells and characterizing them phenotypically. Furthermore, work is ongoing in terms of investigating other segments of the Gastrointestinal Tract (GIT) (systematic study) from dogs suffering with visceral leishmaniasis.
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Full Protocol Obtaining cells from colon of dog with leishmaniasis for flow cytometric analysis
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Method Article
Wagner Luiz Tafuri
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil.
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 15 Dec, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In dogs, a parasite burden is evident throughout the gastrointestinal tract but not is observed pathological alterations. Our aim was isolating lamina propria cells from dogs infected with leishmaniasis to further investigate if the parasite in the gut is correlated with the pathogenesis of the visceral infection. The biopsies were obtained of dogs and incubated with collagenase II. In the cells suspension was added antibodies against superficial and intracellular markers. Few studies of the markers used in this work have been done in the lamina propria cells in leishmaniasis. It is of great importance to determine markers in this tissue especially those who are described as being involved with the persistence of Leishmania infection within cells due to the fact that the infection in the gut does not induce lesions. Furthermore, work is ongoing in terms of investigating other segments of the GIT, a systematic study, from dogs with Leishmania
A pdf of the full version of this protocol can be found "here":http://www.nature.com/protocolexchange/system/uploads/2040/original/Fullprotocol-_protex.2011.273.pdf?1323950759
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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