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Method Article
Radioisotopic Assay of L-carnitine (CN)
Wei Zou
Zou's lab, DTSC, California State Government
Corresponding Author
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L-Carnitine + [1-14C]acetyl-CoA -----> [14C]acetyl-Carnitine + CoASH
Sodium tetrathionate is used to remove CoASH by oxidation so that the reaction is toward the right direction. Then, [14C]acetyl-carnitine is separated from [1-14C]acetyl-CoA by passing the mixture through an anion-exchange resin AG2-X8 (5g resin/100 mL sample). The isotope content in the elute ([14C]acetyl-carnitine) is determined by a scintillation counter.
Keywords
L-carnitine
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This is a preprint. It has not completed peer review.
Method Article
Radioisotopic Assay of L-carnitine (CN)
Wei Zou
Zou's lab, DTSC, California State Government
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 25 Jul, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
L-Carnitine + [1-14C]acetyl-CoA -----> [14C]acetyl-Carnitine + CoASH
Sodium tetrathionate is used to remove CoASH by oxidation so that the reaction is toward the right direction. Then, [14C]acetyl-carnitine is separated from [1-14C]acetyl-CoA by passing the mixture through an anion-exchange resin AG2-X8 (5g resin/100 mL sample). The isotope content in the elute ([14C]acetyl-carnitine) is determined by a scintillation counter.