Radioisotopic Assay of L-carnitine (CN)
L-Carnitine + [1-14C]acetyl-CoA -----> [14C]acetyl-Carnitine + CoASH
Sodium tetrathionate is used to remove CoASH by oxidation so that the reaction is toward the right direction. Then, [14C]acetyl-carnitine is separated from [1-14C]acetyl-CoA by passing the mixture through an anion-exchange resin AG2-X8 (5g resin/100 mL sample). The isotope content in the elute ([14C]acetyl-carnitine) is determined by a scintillation counter.
Posted 25 Jul, 2011
Radioisotopic Assay of L-carnitine (CN)
Posted 25 Jul, 2011
L-Carnitine + [1-14C]acetyl-CoA -----> [14C]acetyl-Carnitine + CoASH
Sodium tetrathionate is used to remove CoASH by oxidation so that the reaction is toward the right direction. Then, [14C]acetyl-carnitine is separated from [1-14C]acetyl-CoA by passing the mixture through an anion-exchange resin AG2-X8 (5g resin/100 mL sample). The isotope content in the elute ([14C]acetyl-carnitine) is determined by a scintillation counter.
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