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Method Article
Micropatterning on glass with deep UV
Nicolas CARPI
Piel Lab, Institut Curie
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method to print micropatterns on glass with extra-cellular matrix proteins to promote cell adhesion. The non-adhesive part is made with polylysine grafted polyethyleneglycol (PLL-g-PEG). This technique is reproducible, cheap, fast and can achieve high resolution (~1 µm).
Keywords
micropatterns, glass, deep-uv, cells, fibronectin, adhesion, pll-g-peg, patterns
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Associated Publications
External forces control mitotic spindle positioning
by Jenny Fink, Nicolas Carpi, Timo Betz, +8
Nature Cell Biology (01 July, 2011)
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This is a preprint. It has not completed peer review.
Method Article
Micropatterning on glass with deep UV
Nicolas CARPI
Piel Lab, Institut Curie
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Jul, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method to print micropatterns on glass with extra-cellular matrix proteins to promote cell adhesion. The non-adhesive part is made with polylysine grafted polyethyleneglycol (PLL-g-PEG). This technique is reproducible, cheap, fast and can achieve high resolution (~1 µm).
Figure 1
Figure 2
Figure 3
Associated Publications
External forces control mitotic spindle positioning
by Jenny Fink, Nicolas Carpi, Timo Betz, +8
Nature Cell Biology (01 July, 2011)