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Method Article
Nicolas CARPI
Piel Lab, Institut Curie
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method to print micropatterns on glass with extra-cellular matrix proteins to promote cell adhesion. The non-adhesive part is made with polylysine grafted polyethyleneglycol (PLL-g-PEG). This technique is reproducible, cheap, fast and can achieve high resolution (~1 µm).
Keywords
micropatterns, glass, deep-uv, cells, fibronectin, adhesion, pll-g-peg, patterns
Figure 1
Figure 2
Figure 3
Matthieu Piel is in one of the founder of Cytoo, a commercial organization selling micropatterned substrates for cell culture.
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Posted 07 Jul, 2011
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Associated Publications
External forces control mitotic spindle positioning
by Jenny Fink, Nicolas Carpi, Timo Betz, +8
Nature Cell Biology (01 July, 2011)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Nicolas CARPI
Piel Lab, Institut Curie
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 07 Jul, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method to print micropatterns on glass with extra-cellular matrix proteins to promote cell adhesion. The non-adhesive part is made with polylysine grafted polyethyleneglycol (PLL-g-PEG). This technique is reproducible, cheap, fast and can achieve high resolution (~1 µm).
Figure 1
Figure 2
Figure 3
Matthieu Piel is in one of the founder of Cytoo, a commercial organization selling micropatterned substrates for cell culture.
Loading...
Associated Publications
External forces control mitotic spindle positioning
by Jenny Fink, Nicolas Carpi, Timo Betz, +8
Nature Cell Biology (01 July, 2011)