This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Nicolas CARPI
Piel Lab, Institut Curie
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method to print micropatterns on glass with extra-cellular matrix proteins to promote cell adhesion. The non-adhesive part is made with polylysine grafted polyethyleneglycol (PLL-g-PEG). This technique is reproducible, cheap, fast and can achieve high resolution (~1 µm).
Keywords
micropatterns, glass, deep-uv, cells, fibronectin, adhesion, pll-g-peg, patterns
Figure 1
Figure 2
Figure 3
Matthieu Piel is in one of the founder of Cytoo, a commercial organization selling micropatterned substrates for cell culture.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 07 Jul, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
External forces control mitotic spindle positioning
by Jenny Fink, Nicolas Carpi, Timo Betz, +8
Nature Cell Biology (01 July, 2011)
Method Article
Nicolas CARPI
Piel Lab, Institut Curie
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 07 Jul, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method to print micropatterns on glass with extra-cellular matrix proteins to promote cell adhesion. The non-adhesive part is made with polylysine grafted polyethyleneglycol (PLL-g-PEG). This technique is reproducible, cheap, fast and can achieve high resolution (~1 µm).
Figure 1
Figure 2
Figure 3
Loading...
Associated Publications
External forces control mitotic spindle positioning
by Jenny Fink, Nicolas Carpi, Timo Betz, +8
Nature Cell Biology (01 July, 2011)