Frozen Permanents of E. coli Cultures
Simple directions to prepare frozen permanents of E. coli or other bacterial cultures that can be stored for years at -80°C.
This is a simple protocol for preparing frozen permanents of E. coli or other bacterial cells. The frozen permanents can be stored for years at -80°C.
100% w/v glycerol sterile filtered (per 100ml: weigh 100g. of glycerol, add water to bring
volume to 100ml. Then sterile filter)
Sterile screw top freezer tubes
Ampicillin or other appropriate antibiotic
Sterile test tubes
Ice bucket with dry ice
Box for -80°C freezer
Overnight to grow culture plus about 15 minutes to complete the rest of the protocol.
Frozen permanents should be good for years if stored at -80°C.
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Posted 14 Jun, 2011
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