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Method Article
Rampyari Walia
Targefect Transfection Group, Targeting Systems
Corresponding Author
License:
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The Targefect-Raw reagent is combined with the Virofect enhancer for optimal transfection of Raw 264.7 macrophages and other macrophage cell lines. The Targefect -Raw reagent enhances gene transfer by allowing the trasnfection complexes to escape degradation in the lysosomes. The Virofect enhancer is a unique adenovirus-derived formulation which greatly enhances the efficeincy of gene transfer by exploiting adenoviral receptors on the cell for efficient intracellular delivery of DNA containing trasnfection complexes. The efficiency of gene transfer into Raw 264.7 cells (Mouse Leukaemic Monocyte Macrophage Cell Line) is around 50-60%.
Keywords
Raw 264.7 transfection, macrophage transfection,
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Rampyari Walia
Targefect Transfection Group, Targeting Systems
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 09 Jun, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The Targefect-Raw reagent is combined with the Virofect enhancer for optimal transfection of Raw 264.7 macrophages and other macrophage cell lines. The Targefect -Raw reagent enhances gene transfer by allowing the trasnfection complexes to escape degradation in the lysosomes. The Virofect enhancer is a unique adenovirus-derived formulation which greatly enhances the efficeincy of gene transfer by exploiting adenoviral receptors on the cell for efficient intracellular delivery of DNA containing trasnfection complexes. The efficiency of gene transfer into Raw 264.7 cells (Mouse Leukaemic Monocyte Macrophage Cell Line) is around 50-60%.
Figure 1
Figure 2
None
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