This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) on chromosomes in suspension for analysis of repetitive DNA by flow cytometry
Peter M. Lansdorp
Lansdorp Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method developed in our laboratory to analyze repetitive DNA in chromosomes using fluorescence in situ hybridization (FISH) and flow cytometry (chromosome flow FISH or CFF). Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition.
Keywords
chromosome, suspension, FISH, PNA, chromosome flow FISH, flow cytometry, probes, fluorescence
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 May, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Analysis of repetitive DNA in chromosomes by flow cytometry
by Julie Brind'Amour and Peter M Lansdorp
Nature Methods (02 May, 2011)
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) on chromosomes in suspension for analysis of repetitive DNA by flow cytometry
Peter M. Lansdorp
Lansdorp Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 May, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method developed in our laboratory to analyze repetitive DNA in chromosomes using fluorescence in situ hybridization (FISH) and flow cytometry (chromosome flow FISH or CFF). Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Associated Publications
Analysis of repetitive DNA in chromosomes by flow cytometry
by Julie Brind'Amour and Peter M Lansdorp
Nature Methods (02 May, 2011)