This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Brian Jester
Université de Strasbourg
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol provides a means to define the level of aminoacylation for a specific tRNA in vivo.
Keywords
tRNA
The authors declare no competing financial interests
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
protocol_pdf tRNA Northern
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 03 May, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
Associated Publications
Stationary-phase expression and aminoacylation of a transfer-RNA-like small RNA
by Sandro F Ataide, Brian C Jester, Kevin M Devine, +1
EMBO reports (26 April, 2011)
The T box regulatory element controlling expression of the class I lysyl-tRNA synthetase of Bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-tRNAAsn
by Niall Foy, Brian Jester, Gavin C Conant, +1
BMC Microbiology (26 April, 2011)
A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine
by D. G. Longstaff, R. C. Larue, J. E. Faust, +4
Proceedings Of The National Academy Of Sciences (26 April, 2011)
Method Article
Brian Jester
Université de Strasbourg
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 03 May, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol provides a means to define the level of aminoacylation for a specific tRNA in vivo.
Loading...
Associated Publications
Stationary-phase expression and aminoacylation of a transfer-RNA-like small RNA
by Sandro F Ataide, Brian C Jester, Kevin M Devine, +1
EMBO reports (26 April, 2011)
The T box regulatory element controlling expression of the class I lysyl-tRNA synthetase of Bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-tRNAAsn
by Niall Foy, Brian Jester, Gavin C Conant, +1
BMC Microbiology (26 April, 2011)
A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine
by D. G. Longstaff, R. C. Larue, J. E. Faust, +4
Proceedings Of The National Academy Of Sciences (26 April, 2011)