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Method Article
Sergey Krylov
York University
Corresponding Author
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Protein isolation from cell lysate can represent a significant challenge. The most efficient way to purify a protein is through the use of target-specific affinity ligands. Development of such affinity ligands, in most cases requires the availability of the target protein in a pure form. Thus, isolating the protein from the cell lysate requires affinity ligands, while developing affinity ligands requires a pure protein. Aptamer-facilitated Protein Isolation from Cells (AptaPIC) resolves this loop problem. AptaPIC is a technique that allows the development of aptamers for a protein in a context of a cell lysate, without any prior purification steps, and the subsequent utilization of the developed aptamers for protein isolation from the cell lysate.
Keywords
Aptamer, protein purification, affinity ligand, affinity purification, tag-free purification
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The authors declare no competing financial interests.
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Associated Publications
Selection of aptamers for a protein target in cell lysate and their application to protein purification
by S. Javaherian, M. U. Musheev, M. Kanoatov, +2
Nucleic Acids Research (09 December, 2010)
Selection of aptamers for a non-DNA binding protein in the context of cell lysate
by Mirzo Kanoatov, Sahar Javaherian, and Sergey N. Krylov
Analytica Chimica Acta (09 December, 2010)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Sergey Krylov
York University
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 13 Jan, 2011
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Protein isolation from cell lysate can represent a significant challenge. The most efficient way to purify a protein is through the use of target-specific affinity ligands. Development of such affinity ligands, in most cases requires the availability of the target protein in a pure form. Thus, isolating the protein from the cell lysate requires affinity ligands, while developing affinity ligands requires a pure protein. Aptamer-facilitated Protein Isolation from Cells (AptaPIC) resolves this loop problem. AptaPIC is a technique that allows the development of aptamers for a protein in a context of a cell lysate, without any prior purification steps, and the subsequent utilization of the developed aptamers for protein isolation from the cell lysate.
Figure 1
Figure 2
Figure 3
The authors declare no competing financial interests.
Loading...
Associated Publications
Selection of aptamers for a protein target in cell lysate and their application to protein purification
by S. Javaherian, M. U. Musheev, M. Kanoatov, +2
Nucleic Acids Research (09 December, 2010)
Selection of aptamers for a non-DNA binding protein in the context of cell lysate
by Mirzo Kanoatov, Sahar Javaherian, and Sergey N. Krylov
Analytica Chimica Acta (09 December, 2010)