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Method Article
Drosophila aconitase activity and reactivation
xh xh
commented on 16 April, 2007
very useful!
Yuvraj Patil
commented on 08 May, 2013
It would be useful to mention the incubation temperature, e.g., Bubber et al (in Neurochemistry International 2004, 45: 1021 – 28 and presumably this one as well - Annals of Neurology 2005, 57: 695 – 703) reactivate [mouse] aconitase at 37°C. It would also help if other conditions were described, such as if anaerobic conditions were maintained during reactivation (e.g. use of glove bags or replacing air with nitrogen/argon in tubes). I assume this procedure was conducted at room temperature in aerobic conditions, which would require operator to carry out assay immediately to prevent loss of activity in a time dependent manner due to aerial oxidation.
xh xh
commented on 16 April, 2007
very useful!
Yuvraj Patil
commented on 08 May, 2013
It would be useful to mention the incubation temperature, e.g., Bubber et al (in Neurochemistry International 2004, 45: 1021 – 28 and presumably this one as well - Annals of Neurology 2005, 57: 695 – 703) reactivate [mouse] aconitase at 37°C. It would also help if other conditions were described, such as if anaerobic conditions were maintained during reactivation (e.g. use of glove bags or replacing air with nitrogen/argon in tubes). I assume this procedure was conducted at room temperature in aerobic conditions, which would require operator to carry out assay immediately to prevent loss of activity in a time dependent manner due to aerial oxidation.
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Posted 20 Mar, 2007
Community comments: 4
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Subject Areas
Biochemistry
Computational biology and bioinformatics
Cell biology
Developmental biology
Biological techniques
Associated Publications
Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster
by Tong, J.J. et al.
Nature Genetics (28 January, 2007)
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This is a preprint. It has not completed peer review.
Method Article
Drosophila aconitase activity and reactivation
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 20 Mar, 2007
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
xh xh
commented on 16 April, 2007
very useful!
Yuvraj Patil
commented on 08 May, 2013
It would be useful to mention the incubation temperature, e.g., Bubber et al (in Neurochemistry International 2004, 45: 1021 – 28 and presumably this one as well - Annals of Neurology 2005, 57: 695 – 703) reactivate [mouse] aconitase at 37°C. It would also help if other conditions were described, such as if anaerobic conditions were maintained during reactivation (e.g. use of glove bags or replacing air with nitrogen/argon in tubes). I assume this procedure was conducted at room temperature in aerobic conditions, which would require operator to carry out assay immediately to prevent loss of activity in a time dependent manner due to aerial oxidation.
xh xh
commented on 16 April, 2007
very useful!
Yuvraj Patil
commented on 08 May, 2013
It would be useful to mention the incubation temperature, e.g., Bubber et al (in Neurochemistry International 2004, 45: 1021 – 28 and presumably this one as well - Annals of Neurology 2005, 57: 695 – 703) reactivate [mouse] aconitase at 37°C. It would also help if other conditions were described, such as if anaerobic conditions were maintained during reactivation (e.g. use of glove bags or replacing air with nitrogen/argon in tubes). I assume this procedure was conducted at room temperature in aerobic conditions, which would require operator to carry out assay immediately to prevent loss of activity in a time dependent manner due to aerial oxidation.
Associated Publications
Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster
by Tong, J.J. et al.
Nature Genetics (28 January, 2007)